Wound Healing Activity of Formulated NipaAlco Gel on Chronic-Binge Ethanol-Fed Male ICR Mice (Mus musculus L.)
DOI:
https://doi.org/10.66133/cv8xcb53Keywords:
citrus reticulata, nypa fruticans, phytochemical, antimicrobial, alcoholismAbstract
The study aimed to assess the wound healing activity of the formulated NipaAlco Gel through in- vitro and in vivo assays. Phytochemical screening of the Citrus reticulata peel extract confirmed the presence of secondary metabolites alkaloids, flavonoids, phenols, saponins, and tannins. Gel formulations (NipaAlcoMHX and NipaAlcoCitro) were prepared in various concentrations. Tests for antimicrobial activity using Cup-plate method, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and Minimum Fungicidal Concentration (MFC) were performed. Antimicrobial potency was evident in that the products demonstrated a mean zone diameter of inhibition ranging from 0-31mm against Staphylococcus aureus, Escherichia coli, and Candida albicans. MIC revealed the best concentration that inhibits microbial growth through turbidity test. MBC and MFC tests showed that the 3% formulation for both NipaAlcoCitro and NipaAlcoMHX Gels is the most optimal concentration that killed the microorganisms, providing evidence that both products have bactericidal and fungicidal properties, important in wound healing. Subsequently, in in-vivo, increasing trend on the qualitative and quantitative assessments for wound healing were observed among all four treatments. Negative control (untreated), however, failed to reach scar formation. Scar formation is an indicator of complete healing within the 30d observation period. Furthermore, histological examination using semi-quantitative approach depicted that the skin tissue treated with NipaAlcoCitro and NipaAlcoMHX Gels exhibited fair to good wound healing. The results are comparable to that of positive control, Povidone-Iodine Ointment. Therefore, it can be inferred that the formulated gel products’ wound healing activities in chronic-binge ethanol-fed ICR mice are as effective as the commercially available one.
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